Genetic Engineering
Price: 2200.00 INR
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ISBN:
9780195696578
Publication date:
08/07/2009
Paperback
760 pages
277.0x215.0mm
Price: 2200.00 INR
We sell our titles through other companies
Disclaimer :You will be redirected to a third party website.The sole responsibility of supplies, condition of the product, availability of stock, date of delivery, mode of payment will be as promised by the said third party only. Prices and specifications may vary from the OUP India site.
ISBN:
9780195696578
Publication date:
08/07/2009
Paperback
760 pages
277.0x215.0mm
Suitable for: Genetic Engineering is designed to serve as a textbook for the students of biotechnology,
Rights: World Rights
Description
Genetic Engineering is designed to serve as a textbook for the students of biotechnology, life sciences, genetics, microbiology, biochemistry, and other related areas. Replete with comprehensive details, it will also serve as a reference for the research students in this field.
Divided into four parts, the book provides in-depth coverage of all major topics in this area. The first part on fundamentals of genetic engineering includes concepts, such as gene cloning, enzymes used in genetic engineering, synthesis of nucleic acids, and techniques like gel electrophoresis and polymerase chain reaction.
The second part on vectors used in gene cloning covers the basic biology of plasmids, ë phages, other cloning vectors, and the strategies adopted for their application in gene cloning. In the third part, generation and screening of recombinants are described. This part includes detailed discussions on DNA ligation, DNA delivery methods, construction of genomic and cDNA libraries, and screening of transformants.
The fourth part on applied genetic engineering covers DNA and protein sequencing, molecular markers, gene silencing, antisense RNA technology, and in vitro mutagenesis. Safety regulations related to genetically modified organisms (GMOs) are also discussed.
Written in a lucid style, the book is highly interactive, which will help readers understand the contents through visualization of concepts and techniques, and their practice.
Table of contents
PART I: FUNDAMENTALS OF GENETIC ENGINEERING
Chapter 1. An Introduction to Gene Cloning
Chapter 2. Enzymes Used in Genetic Engineering
Chapter 3. Restriction Endonucleases
Chapter 4. Chemical Synthesis of Oligonucleotides
Chapter 5. Synthesis of Complementary DNA
Chapter 6. Techniques for Nucleic Acid Analysis and Size Fractionation
Chapter 7. Polymerase Chain Reaction
PART II : VECTORS USED IN GENE CLONING
Chapter 8. Plasmids
Chapter 9. Bacteriophage
Chapter 10. M13 Bacteriophage
Chapter 11. Yeast Cloning Vectors
Chapter 12. Vectors Other Than E. coli Plasmids, A. Bacteriophage, and M13
PART III : GENERATION AND SCREENING OF RECOMBINANTS
Chapter 13. Joining of DNA Fragments
Chapter 14. Introduction of DNA into Host Cells
Chapter 15. Construction of Genomic and cDNA Libraries
Chapter 16. Techniques for Selection, Screening, and Characterization of Transformants
PART IV: APPLIED GENETIC ENGINEERING
Chapter 17. Other Techniques for Genetic Manipulation
Chapter 18. Molecular Markers
Chapter 19. Techniques Used in Genomics and Proteomics
Chapter 20. Applications of Cloning and Gene Transfer Technology
Chapter 21. Safety Regulations Related to Genetic Engineering
Features
- Presents a detailed discussion on in silico drug designing and other bio-algorithms such as BLAST and FASTA
- Discusses applicative and advanced topics such as protein modelling, docking, molecular dynamics, biochemical network, and molecular phylogeny
- Discusses commercial bioinformatics software modules such as Insight II and GCG
- Provides a multiple choice question bank at the end of the book to test the understanding of students
- Includes appendices giving a select list of bio-companies in India, biodatabases with their websites, and a glimpse of the emerging research areas in the subject
Description
Genetic Engineering is designed to serve as a textbook for the students of biotechnology, life sciences, genetics, microbiology, biochemistry, and other related areas. Replete with comprehensive details, it will also serve as a reference for the research students in this field.
Divided into four parts, the book provides in-depth coverage of all major topics in this area. The first part on fundamentals of genetic engineering includes concepts, such as gene cloning, enzymes used in genetic engineering, synthesis of nucleic acids, and techniques like gel electrophoresis and polymerase chain reaction.
The second part on vectors used in gene cloning covers the basic biology of plasmids, ë phages, other cloning vectors, and the strategies adopted for their application in gene cloning. In the third part, generation and screening of recombinants are described. This part includes detailed discussions on DNA ligation, DNA delivery methods, construction of genomic and cDNA libraries, and screening of transformants.
The fourth part on applied genetic engineering covers DNA and protein sequencing, molecular markers, gene silencing, antisense RNA technology, and in vitro mutagenesis. Safety regulations related to genetically modified organisms (GMOs) are also discussed.
Written in a lucid style, the book is highly interactive, which will help readers understand the contents through visualization of concepts and techniques, and their practice.
Read MoreTable of contents
PART I: FUNDAMENTALS OF GENETIC ENGINEERING
Chapter 1. An Introduction to Gene Cloning
Chapter 2. Enzymes Used in Genetic Engineering
Chapter 3. Restriction Endonucleases
Chapter 4. Chemical Synthesis of Oligonucleotides
Chapter 5. Synthesis of Complementary DNA
Chapter 6. Techniques for Nucleic Acid Analysis and Size Fractionation
Chapter 7. Polymerase Chain Reaction
PART II : VECTORS USED IN GENE CLONING
Chapter 8. Plasmids
Chapter 9. Bacteriophage
Chapter 10. M13 Bacteriophage
Chapter 11. Yeast Cloning Vectors
Chapter 12. Vectors Other Than E. coli Plasmids, A. Bacteriophage, and M13
PART III : GENERATION AND SCREENING OF RECOMBINANTS
Chapter 13. Joining of DNA Fragments
Chapter 14. Introduction of DNA into Host Cells
Chapter 15. Construction of Genomic and cDNA Libraries
Chapter 16. Techniques for Selection, Screening, and Characterization of Transformants
PART IV: APPLIED GENETIC ENGINEERING
Chapter 17. Other Techniques for Genetic Manipulation
Chapter 18. Molecular Markers
Chapter 19. Techniques Used in Genomics and Proteomics
Chapter 20. Applications of Cloning and Gene Transfer Technology
Chapter 21. Safety Regulations Related to Genetic Engineering
